From left: Kazakh cosmonaut Aidyn Aimbetov, Roscosmos spacecraft commander Sergei Volkov and ESA astronaut Andreas Mogensen after their last press conference before their launch into space on 2 September from Baikonur, Kazakhstan.
Watch the launch coverage from 03:35 GMT (05:35 CEST) 2 September, with liftoff of their Soyuz TMA-18M spacecraft due at 04:38 GMT (06:38 CEST).
See more pictures from the trios last days on Earth in the image gallery here.
Follow the whole mission with live updates via the iriss mission blog and on Twitter via @esaoperations
Connect with Andreas at: andreasmogensen.esa.int