ESA astronaut Paolo Nespoli will be undertaking experiments as part of the Esperia mission though these are not shown in the timeline below. The following timeline is also subject to change.
 
Flight Day 1 - Launch

• Launch
• Checkout of Shuttle robotic arm
• Switch on Node 2 'Launch-to-Activation' Heaters

• Shuttle heat shield check using robotic arm
• EVA preparations including EVA suit checks
• ISS rendezvous preparations

• Rendezvous and docking including Shuttle backflip manoeuvre
• Preparations for EVA 1
• Unberth robotic arm extension to provide clearance for unberthing Node 2 during EVA 1
• EVA astronauts 'campout' in Quest Airlock to help remove nitrogen from bloodstreams

• Retrieve and stow S-band Antenna Support Assembly; Detach Node 2 heater cables and remove cover from Node 2 aft berthing mechanism; Stow grapple fixture on Node 2; Disconnect Z1/P6 truss fluid lines; Install shrouds on P6 radiator and Sequential Shunt Unit.
• Move and attach Node 2 to Node 1 left-hand docking port with Station robotic arm.
• Pressurise area between Node 1 and Node 2 hatches, perform leak check, open Node 1 hatch.

• Attach cabling between Node 1 and Node 2 and activate shell heaters
• Enter and set up Node 2, establish ventilation
• Shuttle heat shield survey
• EVA 2 preparations
• EVA astronauts 'campout' in airlock

• Disconnect Z1 to P6 truss cables; Unbolt P6; Outfit outside of Node 2 with handrails, worksite interface fixtures, gap spanners and install thermal covers; Remove Node 2 berthing mechanism restraints; Install grapple fixture on Node 2; Configure squib firing unit on S1 truss for deploying radiators; Configure router and replace failed circuit breaker on S0 truss.
• Manoeuvre P6 truss with Station robotic arm to handoff position
• Install Centerline Berthing Camera in Node 2
• Install portable breathing apparatus and fire extinguisher inside Node 2

• Deploy thermal control radiators on S1 truss
• Hand P6 truss from Station robotic arm to Shuttle robotic arm, move Mobile Transporter to end of truss, hand P6 truss back to Station robotic arm
• Node 2 outfitting
• EVA 3 preparations
• EVA astronauts 'campout' in airlock

• Bolt P6 truss to P5 truss, attach P5 to P6 cabling, remove P6 radiator restraint and cover on Sequential Shunt unit and deploy radiator, relocate main Bus Switching Unit from Shuttle cargo bay to External Stowage Platform 2
• Deploy P6 solar arrays
• Node 2 Outfitting

• Shuttle/ISS transfer operations
• EVA 4 preparations
• EVA astronauts 'campout' in airlock
• Crew conference

• Further inspection of Solar Alpha Rotary Joint

• EVA 5 preparations
• EVA astronauts ‘campout’ in airlock

• Disconnect and stow cables from Destiny to Pressurised Mating Adapter 2; Remove cover on Node 2 forward docking mechanism, demate avionics cables, and release fluid tray bolts; Temporary stow CETA light on Destiny; Configure cables for power transfer for S0 truss/Node 1/Zvezda and Pressurised Mating Adapter 1/Zarya. Retrieve Baseband Signal Processor from Z1 truss. Release bolts on P1 Nitrogen tank Assembly

• Undocking
• Station flyaround
• Shuttle heat shield survey

• Landing preparations

• Landing preparations
• Deorbit
• Landing