In vitro simulation of spaceflight environment to elucidate combined effect of altered gravity, ionizing radiation, and psychological stress on dermal fibroblast

Eline Radstake

Belgian Nuclear Research Centre SCK CEN & Ghent University

Long-term exposure to microgravity, ionizing radiation and increased levels of psychological stress can cause changes in the astronauts’ skin, resulting in skin rashes, itches and delayed wound healing during space missions. There is still a lack of understanding how the complex spaceflight environment induces these defects. This PhD project aims to investigate how exposure to a combination of spaceflight stressors can affect the structure and function of the skin, and how they can hamper wound healing. For this we have developed in vitro simulation models and are exposing primary human dermal fibroblasts to hydrocortisone, ionizing radiation and simulated microgravity. Results indicate a significant negative effect of hydrocortisone as well as simulated microgravity on wound healing capability of dermal fibroblasts. Furthermore, a project has been initiated with the support of the European Space Agency Academy “Spin Your Thesis!” Campaign, aiming to investigate the effects of an increased gravitational force on fibroblast function related to wound healing. Altogether the results of this PhD project will give more insights into the effects of combined spaceflight stressors on dermal skin cells, and improve risk assessment for human deep space exploration.