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DNA extraction from a Kiwi fruit
DNA Extraction from Kiwi Fruit
 
Subject Chemistry
Level Upper secondary
Language English
Type Experiment

 
 
Experiment
 
A simple protocol to see and touch real DNA:

  • cut the kiwi (1/2 big or 1 small) into small pieces
  • take the table salt, the washing-up liquid, and the distilled water and mix it well in a beaker
  • add the chopped kiwi
  • wait for 10 minutes
  • blend the mixture (with a blender) for exactly 5 seconds
  • filter the mixture into a second beaker
  • add 10 drops of protease (meat tenderizer or contact lens cleaning liquid) and mix
  • pour 20 ml of the filtrate into a large test tube
  • very carefully pour ice-cold ethanol (95%) down the side of the tube to form a layer on top of the kiwi extract
  • collect the filaments of DNA wrapping them around a inoculation loop.

 
 
Contact details
 
NameImmacolata ErcolinoGiulia Realdon
Emailimmacolata.ercolino@istruzione.itgiulia.realdon@virgilio.it
 
 
 


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