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DNA extraction from a Kiwi fruit
DNA Extraction from Kiwi Fruit
Subject Chemistry
Level Upper secondary
Language English
Type Experiment

A simple protocol to see and touch real DNA:

  • cut the kiwi (1/2 big or 1 small) into small pieces
  • take the table salt, the washing-up liquid, and the distilled water and mix it well in a beaker
  • add the chopped kiwi
  • wait for 10 minutes
  • blend the mixture (with a blender) for exactly 5 seconds
  • filter the mixture into a second beaker
  • add 10 drops of protease (meat tenderizer or contact lens cleaning liquid) and mix
  • pour 20 ml of the filtrate into a large test tube
  • very carefully pour ice-cold ethanol (95%) down the side of the tube to form a layer on top of the kiwi extract
  • collect the filaments of DNA wrapping them around a inoculation loop.

Contact details
NameImmacolata ErcolinoGiulia Realdon

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